Influence of UV on Gadd45?/? in human hepatoma carcinoma cell HepG2 / 临床检验杂志

ABSTRACT

Objective To investigate the change of Gadd45?/? in HepG2 cells after UV radiation.Methods HepG2 cells were cultured in vitro followed by radiation with UV (254nm,10 J/m2)and harvested at 1,2,4 h respectively.The induction of Gadd45?/? mRNA by UV was analyzed using RT-PCR,and the induction of Gadd45?/? protein was determined by Western blot assay at the given interval simultaneously.Results The peak values of Gadd45?/? mRNA appeared at 4 h and 1h after induction respectively,while the both of the peak value of Gadd45?/? protein were found at 1h after UV radiation.Conclusion Gadd45?/? could be rapidly induced by UV radiation in HepG2 cells.