The effect of CD151 on Akt-Ser~(473) phosphorylation and human umbilical veine endothelial cell proliferation / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-588690
ABSTRACT
Objective:
To investigate the the effect of CD151 on Akt-Ser473 and human umbilical veine endothelial cell proliferation.Methods:
To construct pAAV-CD151 and transiently transfect to HUVEC(human umbilical veine endothelical) mediated with Lipofectamine 2000.HUVEC were assigned to control group,GFP group,CD151 group.After transfection,the expression of CD151,Akt and phospho-Akt were measured by western blot.Proliferation of HUVEC were measured by MTT colorimetric assay.Results:
①The expression of CD151,phospho-Akt of pAAV-CD151 transfected group were significantly increased compared with non-transfected group and pAAV-GFP transfected group(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Postgraduates
Year:
2003
Type:
Article
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