Effects of transforming growth factor 1 and hypoxia on the expression of vascular endothelial growth factor for Hepatoma cells / 基础医学与临床
Basic & Clinical Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-588839
ABSTRACT
Objective To observe the effect of Vascular Endothelial Growth Factor(VEGF) to the expression in hepatoma cells affected by TGF-?1 and hopxia. Methods HepG2 cells were cultured in vitro, and treated with different doses of TGF-?1 and Cobalt chloride hexahydrate(CoCl2), or with TGF-?1 and CoCl2. The change of VEGF protein and mRNA was detected by immunohistochemistry and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results Immunohistochemistry and RT-PCR showed that the expression of VEGF protein and mRNA were significantly higher in TGF-?1 groups or CoCl2 groups than that of control group(P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2006
Type:
Article
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