Study on immunosensor of immobilized HRP-HBV antibody on Au colloid / 医疗卫生装备

ABSTRACT

Objective To form Hepatitis B electrochemical immunosensor for the clinic detection. Methods Using the gold electrode as the fundus,the thionine and the HBV horseradish peroxidase(HRP) antibody were electrostaticly bounded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immunosensor. Cyclic voltammetry and linear scan voltammetry were used to investigate the chemical characteristics of the immunosensor. Results The linearity of Hepatitis B in the range of 130～1200 with the correlation coefficient of 0. 994 was obtained. Conclusion The Hepatitis B electrochemical immunosensor formed by this way can be used to the clinic detection.