Regulation of Jagged1 expression by its receptor Notch1 in prostate cancer PC3 cells / 基础医学与临床

ABSTRACT

Objective To investigate the effect of Notch1 and Jagged1 on the growth of prostate cancer PC3 cells and the regulation of Jagged1 expression by its receptor Notch1 in PC3. Methods Notch1 and Jagged1 expression were inhibited by siRNA interference. The growth rate of PC3 cells was measured by MTT. Notch1 expression was inhibited by siRNA interference and promoted by transfection of Notch1 cDNA. The changes of Notch1 and Jagged1 protein and mRNA levels were measured by Western blot and Real-time RCR. Results The growth of PC3 cells decreased after inhibiting Notch1 and Jagged1 expression. Jagged1 protein level decreased after inhibiting Notch1 expression and increased after over-expressing Notch1. Moreover, the change of Jagged1 protein level was discordant with the situation of mRNA. Conclusion In PC3 cells, Notch1 and Jagged1 are important to cell growth. Notch1 may regulate Jagged1 expression.