Effect of ischemic pretreatment on cPKC isoform-specific membrane translocation and protein expression in retina of rats / 基础医学与临床

ABSTRACT

Objective To identify certain isoforms involved in the onset of retinal ischemic preconditioning (IPC), the effects of ischemic pretreatment (IP) were observed on level of conventional protein kinase C (cPKC) ?,?Ⅰ、?Ⅱand ? isoform-specific membrane translocation and protein expression in retina of rats. Methods Retinal IP was produced by intra-ocular pressure (IOP) elevation for 5 minutes in anesthetized Wistar rats. Sham operation was similar to IP except the pressure elevation. 10, 20, or 40 minutes and 1, 12, 24, 72 or 168 hours after the procedure, cPKC isoform-specific membrane translocation and protein expression were analyzed using Western-blot. Results cPKC? protein expression significantly increased from 12 h to 168 h. A peak reached at 72 h after IP. cPKC? membrane translocation enhanced during 20 min to 1 hours with a peak at 40 min. cPKC? protein expressionlevels significantly increased from 12 hours to 72 hours. A peak was found at 24 hours after IP. However, there were no significant changes in both membrane translocation of cPKC?, ?Ⅰ、?Ⅱand protein expression of cPKC?Ⅰ, ?Ⅱ in retina of rats following IP.Conclusion The enhanced cPKC? membrane translocation, the increased cPKC? and ? protein expressions might be involved in the onset and sustain of retinal IPC in rats respectively.