Expression of 1200009K10 gene in retina of mice

Huangxuan SHEN; Qingjiong ZHANG

ABSTRACT

BACKGROUND:

1200009K10 gene is from the lung cDNA pool of adult mouse and announced in January 2002, but its function is still not known.The predicted protein has five domains Kelch motif, Kelch domain, BTB/POZ domain, BTB domain and β propeller domain, all of which are involved in protein-protein interactions and some enzymatic activities.

OBJECTIVE:

To explore the correlation between the expression of 1200009K10 gene and retinitis pigmentosa in mice.

DESIGN:

Observational and comparative trial.

SETTING:

Institute of Zhongshan Ophthalmic Center of Sun Yat-sen University.MATERIALS The rd mice, rds mice and passage mice from C3B mice as normal control were purchased from Jackson Laboratory (Bar Harbor,Maine 04609, USA), and raised in second class unit of Experimental Animal Center of Sun Yat-sen University.

METHODS:

The retinal RNA of mice was extracted and isolated and 1200009K10 gene was amplified by rapid amplification of eDNA ends method (RACE). The gene expressions in the retina of rds, rd and C3B mice were analyzed, respectively by gene-specific real-time quantitative PCR.MAIN OUTCOME

MEASURES:

The expression contents and patterns of 1200009K10 gene in retina of three kinds of mice.

RESULTS:

One clone was obtained after RACE of retinal RNA extracted from retina of mice, which had an almost identical sequence with the 1200009K10 gene. Expression of 1200009K10 gene was classified into 6stages 7, 12, 25, 37, 50 (near sex maturity) and 150 days after born. The expressions of 1200009K10 gene in the retina of rds, rd and C3B mice were low at postnatal day 7 (P7), higher between P7-12, stable between P12-50, and finally increased a little. The expression trend and level of 1200009K10 gene of C3B mice was nearly the same with that of the rds and rd mice, and higher than that of rds and rd mice at P12. Although there were differences in expression levels of 1200009K10 gene among the three kinds of mice, the expression patterns were almost the same.

CONCLUSION:

1200009K10 gene may not participate in the development of retinal degeneration.