Effects of simvastatin and granulocyte colony stimulating factor on vascular reendothelialization after rat carotid artery injury / 中国介入心脏病学杂志

ABSTRACT

Objective To study the effect of endothelial progenitor cells mobilization on vascular endothelial repair after carotid artery injury in rats.Methods The rat balloon-injured carotid model was established.In the control groupsaline was administrated intragastrically;the simvastatin groupsimvastatin 10 mg/(kg?d)was administrated intragastrically and in the simvastatin+ granulocyte colony stimulating factor(G-CSF)groupG-CSF 100 ?g/(kg?d)was intraperitoneally injected togother with intragastrical simvastatin from 1 week before operation to 2 weeks after operation.The repair rate of injuried endothelium,neointima/media area ratio(IA/MA),the positive cell index of proliferating cell nuclear antigen(PCNA)and nitric oxide(NO)release in peripheral blood were detected.In addition,the ratio of CD34+VEGFR2+ double positive expression cell was detected by fluorescence activated cell sorter(FACS),which indicated the level of EPCs.Results The repair rate of injured endothelium was increased(12.3%)in the simvastatin group compared with the control group;IA/MA was reduced 18.4%;NO release was increased 33.9% and the positive cell index of PCNA was reduced 5.7%.These data were 38.2%,50.3%,33.9% and 37.2% respectively in simvastatin+G-CSF group.Compared with the control group,the ratio of CD34+VEGFR2+ double positive expression cell was increased 87.6% in peripheral blood,and it increasd 343.4% in simvastatin+G-CSF group.Conclusion The results suggest the possibility that simvastatin may promote the EPCs from bone marrow to peripheral blood,so it can improve the effect of repair rate of injured endothelium and inhibit neointimal hyperplasia.In addition,G-CSF can enhance the effect of simvastatin on the injured endothelium and neointima hyperplasia.