Efficacy of Wearing Gloves to Protect Laboratory Staffs from Hepatitis B Virus (HBV) Infected Via Hands / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To survey hepatitis B virus(HBV) contamination on hands of laboratory staffs and to evaluate the protection efficacy against HBV infection by wearing gloves.METHODS Samples from used gloves and gloves-protected hands were carried out according to the methods stipulated in the Technical Guidelines for Disinfection.All samples were divided into 3 groupsgloves worn for 1h,gloves worn for 3h and gloves-protected hands,each group had 202 cases.To detect HBsAg and HBV DNA by microparticle enzyme immunoassay(MEIA) and fluorescence quantitative polymerase chain reaction(FQ-PCR),respectively.RESULTS HBsAg polluted rates of gloves worn for 1h,gloves worn for 3h and gloves-protected hands were 22.77%,34.65% and 2.48%,respectively;HBV DNA polluted rates of gloves worn for 1h,gloves worn for 3h and gloves-protected hands were 27.72%,41.58% and 2.48%,respectively.The HBsAg and HBV DNA polluted rates of gloves worn for 3h were higher than those of gloves worn for 1h,the difference was significant between them(P