A Novel Specific Small Molecule Peptide for Non-small Cell Lung Cancer Cell A549 / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-590049
ABSTRACT
To screen small molecule peptide specific binding to non-small cell lung cancer cell (A549) using the "one-bead one-peptide" combinatorial library. Twenty-nine positive beads binding to A549 cell were totally obtained after primary screening. Consensus peptide sequence of -NGXG-was identified by amino acid sequencing in ten beads. Peptide cNGQGEQc was re-synthesized on beads and further studied for its cell specificity, alanine scanning and site-directed deletion. The results showed that cNGQGEQc is specific for cell attachment to non-small cell lung cancer cells including A549, Calu-1 and H178, but not to other tumor cell lines. Both motif of -NGXG-and the length of six peptides are very important for A549 adhesion. Peptide cNGQGEQc labeled with FITC can specifically bind to A549 cell. In a blocking assay with anti-integrin antibodies(?1~6, ?v/?1~5), cell adhesion of A549 to peptide beads was obviously inhibited by integrin ?3 combining with any ? subunits. Results suggested that small molecule peptide cNGQGEQc can bind specifically to non-small cell lung cancer cell A549 via integrin ?3 on cell surface.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Progress in Biochemistry and Biophysics
Year:
2006
Type:
Article
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