Expression of ?-Lactamase in Pseudomonas aeruginosa Biofilms / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To explore production of ?-lactamase induced by antibiotics in Pseudomonas aeruginosa biofilm and planktonic bacteria.METHODS In vitro models of P.aeruginosa biofilm were built up.The planktonic bacteria and P.aeruginosa biofilms were exposed to different concentrations of imipenem and ceftazidime.The quantitative analysis of ?-lactamase was undertaken.The class of ?-lactamase was verified by modified cefoxitin three-dimensional test.RESULTS Both P.aeruginosa biofilm and planktonic bacteria showed significant ?-lactamase activity in the presence of antibiotics.The maximal ?-lactamase activities of both were significantly different with the statistic method of test(P