Development of enzyme-linked immunosorbent assay method for anti-B lymphocyte stimulator autoantibodies and its preliminary clinical application / 医学研究生学报

ABSTRACT

Objective:To investigate the prevalence of the antibodies against the B lymphocyte stimulator(BlyS),(BlyS-Ab) in patients with clinical diseases,and to explore the role of BlyS-Ab in the pathogenesis of autoimmune diseases(AID).Methods:By optimizing experimental conditions,we developed ELISA methods for serum levels of BlyS-Ab,followed by methodological and clinical application assessment and investigation of the relationship of serum BlyS-Ab levels with the titers of anti-nucleus antibodies(ANA) and other autoantibodies.Results:The intra-assay precision,namely the average coefficient values of BlyS-Ab was 7.8%,and the specific inhibition rate for BlyS-Ab by series concentration of BlyS was up to 77.3%.Clinical investigation revealed that the serum levels of BlyS-Ab was significantly higher in patients with autoimmune diseases(including SLE,RA,myasthenia gravis) and other medical cases(such as allergic purpurics,chronic nephritis,etc.) than in the normal control(P