Expression and correlation between erythropoietin mRNA and hypoxia inducible factor-1? mRNA in rats with brain ischemic tolerance / 临床神经病学杂志

ABSTRACT

Objective To research the expression and correlation between erythropoietin(EPO)mRNA and hypoxia inducible factor-1?(HIF-1?)mRNA in rats with brain ischemic tolerance induced by ischemic preconditioning.Methods Models of cerebral ischemic preconditioning and ischemic-reperfusion(preconditioning group)were made by twice suture method,after the short ischemic preconditioning 1 d,3 d,7 d,14 d and 21 d respectively,the models were underwent ischemic-reperfusion again.The infarct volume was measured by tetrazolium chloride(TTC)staining,the expressions of HIF-1? mRNA and EPO mRNA in the different time(1～21 d)of ischemic-reperfusion were detected by reverse transcriptase polymerase chain reaction(RT-PCR)and compared with non-preconditioning group.Results The infarct volume in the 1 d,3 d and 7 d subgroups of preconditioning group were significantly smaller compared with non-preconditioning group(all P