Methodological evaluation on ELISA for detecting concentration of apo A-V in human plasma / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-590201
ABSTRACT
Objective To establish an ELISA to detect the concentration of apo A-V in human plasma and evaluate the sensitivity and stability of the method.Methods The standard curve of recombinant human apo A-V, the lowest detectable concentration, the day-to-day variation and between-plate-within-day variation, the interference of other non-specialized apolipoprotein were examined, and the reference range was established by detecting the plasma concentration of apo A-V in 505 healthy subjects.Results The linear range of the established method was 5.86 - 187.5 ng/ml with lowest detectable concentration of 2.34 ng/ml. The interferences for apo A-V detection due to Lp(a), apo AI and apo B was never found in our study. The day-to-day variation and between-plate-within-day variation were less than 10% respectively.Conclusion An ELISA for detecting the concentration of apo A-V in human plasma was established and the method can serve as a useful tool for research on mechanism and function of apo A-V in TG metabolism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Clinical Laboratory Science
Year:
2006
Type:
Article
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