Your browser doesn't support javascript.
loading
Inhibition of anti-PDGF on proliferation of cultured human retinal pigment epithelial cells in vitro / 吉林大学学报(医学版)
Article in Zh | WPRIM | ID: wpr-590340
Responsible library: WPRO
ABSTRACT
Objective To study the inhibition of anti-PDGF on proliferation of cultured human retinal pigment epithelial cells(hRPE) in vitro.Methods hRPE were cultivated and were exposed to different concentrations of anti-PDGF(0,1?10-6,5?10-6,1?10-5,5?10-5 and 1?10-4 mg?L-1) respectively .Growth curves were measured with cell counting and the vitalities of cells were examined by percentage of vital cells and total cells.Using MTT staining colorimetric to measure the inhibitory rate.The changes of cell cycle of hRPE were collected and their growth were detected with FCM analysis and the morphological changes of cells were observed by light microscope and electron microscope.Results Anti-PDGF of 1?10-6mg?L-1 stimulated hRPE proliferation slightly.AntiPDGF at dosages ranging from 5?10-6mg?L-1 to 1?10-4mg?L-1 inhibited cell proliferation effectively in a dose-dependent and time-dependent manner(P
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Jilin University(Medicine Edition) Year: 2006 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Jilin University(Medicine Edition) Year: 2006 Type: Article