Construction of Survivin specific small interfering RNA expression vector,its antiproliferative and proapoptotic effect on lung adenocarcinoma cells / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-590356
Responsible library:
WPRO
ABSTRACT
Objective To test the antiproliferative and proapoptotic effect of Survivin small interfering RNA(siRNA) expression vector on lung adenocarcinoma cell A549 in vitro.Methods The Survivin-siRNA expression vector was constructed and conformed by sequencing.The inhibitory effect of Survivin-siRNA was tested by fluorescent quantitative reverse transcription polymerase chain reaction(FQ RT-PCR),Western blot and immunohistochemistry.The cell proliferation and apoptotic rate were assayed by tetrazolium bromide(MTT)colorimetry and flow cytometry.Results Survivin-siRNA expression vector was constructed and transfected into A549 cell.It effectively reduced mRNA and protein level of Survivin.Immunohistochemistry also showed lower expression of Survivin.The A549 cells transfected with Survivin-siRNA had a lower cellular growth rate than that of control group(P
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Index:
WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2006
Type:
Article