Impact Factors of Pseudomonas aeruginosa Biofilms Formation / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To compare the difference of Pseudomonas aeruginosa(PAE) biofilms formation in different conditions including incubated time,temperature,attaching materials,and flowing speed.METHODS PAE biofilms were established in a chemostat-coupled MRD and detected with the method of viable bacteria counting.The number of CFU/disc was measured in different culture time(8h,24h or 72h),different temperature(23℃ or 37℃),different adherent materials(glass or silicone),different flow velocities(30ml/h or 90ml/h) and different culture media(M63 medium,M-H croth or LB broth).RESULTS Keeping the other conditions invariable,the log10CFU/disc of viable bacteria in 8h,24h or 72h biofilms were 4.01?0.26,4.59?0.49 or 5.20?0.47,respectively(P