Detection of Extended-spectrum ?-Lactamases and AmpC ?-Lactamases and Analysis of Drug Resistance in Enterobacter cloacae / 中华医院感染学杂志
Chinese Journal of Nosocomiology
;
(24)2006.
Article
in Chinese
| WPRIM
| ID: wpr-590429
ABSTRACT
OBJECTIVE To investigate the genotypes of extended-spectrum ?-lactamases(ESBLs)and drug resistance in Enterobacter cloacae for clinical treatment.METHODS E.cloacae was identified by VITEK-AMS;ESBLs and AmpC ?-lactamases phenotypes were detected by three-dimensional tests.Detection of ?-lactamases resistant genes were performed by PCR.Plasmid conjugation test was used to determine the location and the transmission of encoding genes of ?-lactamases.The antibotic susceptibility of E.cloacae was detected according to the CLSI guideline.RESULTS The incident rate of ESBLs producing E.cloacae was 48.8% in 86 strains.CTX-M was the main type of ESBLs.The detection rate of CTX-M-3,CTX-M-9,CTX-M-14 and SHV-12 genotypes in isolates were 27.9%,16.3%,3.4% and 18.6%,respectively.Strains producing both ESBLs and AmpC ?-lactamases were 11(12.8%).The strains only producing ESBLs or AmpC ?-lactamases were 31(36.0%) and 8(9.3%),respectively.The sensitive rate of ESBLs isolates to imipenem was 100.0%.Eight ESBLs strains were conjugated successfully.CONCLUSIONS CTX-M and SHV-12 are the main genotypes of ESBLs strains.ESBLs genes can be horizontally transferred to recipient by plasmid conjugated test.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Practice guideline
Language:
Chinese
Journal:
Chinese Journal of Nosocomiology
Year:
2006
Type:
Article
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