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Characterization of The Na~+/H~+ Antiporter in Tonoplast Vesicles From Populus tremula. calli / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics ; (12)2006.
Article in Chinese | WPRIM | ID: wpr-590440
ABSTRACT
The Na+/H+ antiporter in vacuolar membranes transports Na+ from the cytoplasm to vacuoles using a pH gradient generated by proton pumps, which could reduce Na+ toxicity. It is uncertain that whether the woody plants have the same mechanism. Through differential centrifugation and sucrose density gradient centrifugation, tonoplast vesicles were isolated from Populus tremula calli broken by blender. After establishing pH gradient by V-ATPase, Na+ could dissipate the pH gradient, which indicates that there is Na+/H+ antiporter in the tonoplast vesicles from Populus tremula calli (Km=11.4 mmol/L). Amiloride could inhibit the Na+/H+ antiporter activity. The antiporter could transport Na+ and K+, the affinity for Na+ is higher. Salt stress decreased Km and Vmax.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Biochemistry and Biophysics Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Progress in Biochemistry and Biophysics Year: 2006 Type: Article