Decreased inhibitory synaptic function in CA1 region of hippocampus of adult rats following global ischemia-reperfusion / 基础医学与临床

ABSTRACT

Objective To observe the effects of global ischemia-reperfusion on inhibitory synaptic function in hippocampal CAl region of adult rats.Methods Animals were randomly divided into three groups sham-operation group(SH),ischemia-reperfusion 3 day(IR-3) and 7 day group(IR-7).Global ischemic episode was achieved by 4-vessel occlusion.Evoked inhibitory postsynaptic currents(eIPSCs) were recorded from CA1 pyramidal cells in hippocampal slices using whole-cell voltage-clamp.Results The eIPSCs amplitudes generated by lower stimulus intensities were significantly decreased in both IR-3 and IR-7 rats as compared with SH rats.Moreover,the rise time of eIPSCs in IR-7 group was significantly decreased as compared with SH group(P