Effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells into neural cells / 临床神经病学杂志
Journal of Clinical Neurology
;
(6)1992.
Article
in Chinese
| WPRIM
| ID: wpr-591006
ABSTRACT
Objective To investigate the effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells (ADSCs) into neural cells. Methods Human ADSCs were isolated from human liposuction tissues and cultured in NIM with different dose of GM1, which was used to induce ADSCs into neural cells. The experiment was separated into six groups control group (?-MEM), Group A (NIM), group B (NIM+50 ?mol/L GM1), group C (NIM+100 ?mol/L GM1), group D (NIM+200 ?mol/L GM1) and group E (NIM+400 ?mol/L GM1). Cells' growth and morphologic changes were observed under invert phase-contrast microscopy. The expressions of Nestin, NSE, MAP2 and GFAP were identified by immunocytochemistry methods.Results (1)ADSCs could differentiate into neural cells in NIM with different dose of GM1. The number of neural cells differentiated from ADSCs increased in a certain period, especially in group E at each time point (all P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Clinical Neurology
Year:
1992
Type:
Article
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