Homocysteine downregulates the expression of estrogen receptor alpha in aorta of ovariectomized rats by methylation modification / 基础医学与临床

ABSTRACT

Objective To observe the effects of homocysteine on the expression of estrogen receptor alpha(ER?) mRNA and the CpG island methylation of ER? gene promotor region in rats.Methods The serum level of homocysteine in rats was measured by reversed-phase HPLC.RT-PCR and methylated specific PCR(MSP) were applied to assay mRNA expression and the methylation status in promoter CpG island of ER? gene in aorta and cultured rat aortic smooth muscle cells(RASMCs).Results The homocysteine level was increased in ove and ove+H groups(P