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Induction of retinoic acid on PC12 cells differentiated into neuron-like cells / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition) ; (6)2006.
Article in Chinese | WPRIM | ID: wpr-591260
ABSTRACT
Objective To study the condition of pheochromocytoma cells(PC12 cells) differentiated into neuron-like cells induced by retinoic acid(RA) and to observe the lengh of neurite and max diameter and the expression of MAP2 in PC12 cells during their differentiation into neuron-like cells.Methods There were seven groups0.1,0.3,0.5,1.0,2.0 and 5.0 mg?L-1RA groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to RA with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to RA for 72 h.Results The number of MAP2 positive cells was significantly increased in 0.3,0.5,1.0,2.0 mg?L-1RA groups compared with control group and the most significant concentration was 1.0 mg?L-1(P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Jilin University(Medicine Edition) Year: 2006 Type: Article