Experimental study on biological character of rabbit lingual keratinocyees / 中国基层医药
Chinese Journal of Primary Medicine and Pharmacy
; (12): 455-457,后插四, 2009.
Article
in Zh
| WPRIM
| ID: wpr-591485
Responsible library:
WPRO
ABSTRACT
Objective To explore the cultural method of lingual keratinocytes and make preparations for further investigation in using lingual keratinocytes as a new choice of seed cells for the reconstruction of tissue engineered corneal epithelium.Methods Keratinocytes were enzymatically dissociated from lingual tissue specimens using 0.25%disepase and 0.05%trypsin.The biological characteristics of the cells were observed through phase microscope.Meanwhile.1ingual kemtinoeytes were immtmohistochemically labeled with broad spectrum keratin antibody (AE1/AF3).Results The cells revealed the form of "road metal" and could be passed for 3 or 4 generations.Immunohistochemistry indicated that lingual keratinocytes were positive for AE1/AE3 staining.Condusion Lingual kemtinocytes of rabbits can be cultured with KSFM in vitm and magnitude quantity can be attained,laying a favorable foundation for lingual keratinocytes as a new choice of seed cells for corneal epithelium reconstruction with tissue engineering.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Primary Medicine and Pharmacy
Year:
2009
Type:
Article