Inhibitory effect of endostatin gene on transplanted osteosarcoma in nude mice / 中国肿瘤生物治疗杂志

ABSTRACT

Objective:To investigate the inhibitory effects of endostatin (ES) on the growth and metastasis of transplanted osteosarcoma UMR106 cells in nude mice. Methods: pBudCE4.1/ES was amplified and transfected into osteosarcoma cell line UMR106 through lipidosome; the non-transfected UMR106 cells were eliminated by Zeocin. The proliferation of ES-UMR106 cells was examined by MTT assay and the production of ES by ES-UMR106 cells was investigated by ELISA. The effects of ES on the in vitro proliferation of vascular endothelial cells were observed by MTT assay. Sixteen nude mice were randomly divided into 2 groups one group was transplanted with UMR106 cells and the other with ES-UMR106 cells. The tumor size, pathological observation, tumor angiogenesis, and the pulmonary metastasis were observed. Results: The proliferation of UMR106 cells was not affected by ES transfection. ES-UMR106 cells expressed bioactive ES, and the ES level in the medium was higher than 350 ng/ml 48 h after transfection. ES produced by ES-UMR106 cells significantly inhibited the proliferation of vascular endothelial cells. Compared with UMR106 transplanted tumor, ES-UMR106 transplanted tumor grew slowly in nude mice, and the formed tumors was well-margined and had less angiogenesis and mass necrosis. Two of the 8 mice transplanted with UMP106 cells had pulmonary metastasis and no metastasis was found in ES-UMR106 transplanted group. Conclusion: Transfection with recombinant endostatin plasmid can inhibit the growth and metastasis of transplanted osteosarcoma in nude mice by inhibiting vascular endothelial cell proliferation.