Mobilization of peripheral blood stem cells in mice with short-duration and high-dose recombinant human granulocyte colony-stimulating factor

Jinsong YAN; Jinsheng TONG; Zhenlan SONG

ABSTRACT

BACKGROUND:

The prompt and efficient peripheral blood stem cell (PBSC) mobilization is necessary for repairing and regenerating injured tissues. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) or its combination with a large dose of chemotherapy is usually utilized for PBSC mobilization, but the regimen requires a long time and a complex procedure.

OBJECTIVE:

To modify the common PBSC mobilization, and investigate mobilization effect of PBSC in KM mice with short-duration and high-dose rhG-CSF. DESIGN, TIME AND

SETTING:

A randomized controlled animal trial was carried out in the laboratory of Hematology, the Second Affiliated Hospital to Dalian Medical University from September 2006 to March 2007. MATERIALS Twenty-four KM male mice of 8 months old were divided into 3 groups according to the administration conventional regimen group, short-duration and high-dose group, and saline control group.

METHODS:

Mice in the conventional regimen group were injected subcutaneously with rhG-CSF (0.2 ?g one time, twice a day for 6 days). Mice in the short-duration and high-dose group were injected with equal volume of normal saline at first 4 days, and then with rhG-CSF (2.5 ?g one time, twice a day for 2 days). Mice in the saline control group were injected subcutaneously with equal volume of normal saline for 6 days. MAIN OUTCOME

MEASURES:

Peripheral blood white blood cell (WBC) and colony-forming unit-granulocyte macrophage in KM mice were counted.

RESULTS:

WBC in the peripheral blood of KM mice increased rapidly in conventional regimen group and short-duration and high-dose group. No obvious change of WBC was observed in the control group. The number of colony-forming unit-granulocyte macrophage in conventional regimen group and short-duration and high-dose group was significantly elevated over 50 folds than control group (P 0.05).

CONCLUSION:

There is a good efficacy and feasibility in PBSC mobilization of KM mice with short-duration and large-dose rhG-CSF, and it is a successful amelioration of PBSC mobilization of mice peripheral blood.