Intramuscular nerve effects on repair of rectus femoris injury / 中国组织工程研究

ABSTRACT

BACKGROUND: Generally, repairing the injuries of sketetal muxcle is to suture the epimysial and exterior muscular nerve. OBJECTIVE: To determine the intramuscular nerve effects on the recovery of lacerated musculus rectus femoris. DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Animal Experimental Center, Chongqing Medical University from September 2006 to August 2007. MATERIALS Twenty New Zealand rabbits weighing 2.0-2.5 kg were used to establish models of right skeletal muscle injury; left side was used as controls. METHODS: Twenty rabbits were randomly divided into two groups. Rabbits in the intact intramuscular nerve group underwent complete muscle laceration with intramuscular nerve intact. Musculus rectus femoris was traversed at 4.0-5.0 mm from nerve entry point. Rabbits in the intramuscular neurotmesis group underwent complete muscle laceration, but intramuscular nerve was cut concomitantly at the same place as above. MAIN OUTCOME MEASURES: 28 weeks after surgery, wet weight and isometric contraction of musculus rectus femoris were detected. Morphological change of musculus rectus femoris was observed by Hematoxylin & Eosin Staining. Fibrous degree was measured by Masson trichrism staining. Myofibrillar type was examined by myosin ATPase staining and tetrazolium staining method of reduced coenzyme Ⅰ to calculate cross-sectional area of muscle fiber. RESULTS: Compared with the controls, wet weight and isometric contraction of musculus rectus femoris reduced in the intramuscular neurotmesis group (P