L-3-n-butylphthalide inhibits expression of nuclear factor-?B and I?B-? in primary cultured murine brain glial cells following oxygen-glucose deprivation/reoxygenation / 基础医学与临床

ABSTRACT

Objective To observe the effect of l-3-n-butylphthalide(l-NBP)on the of nuclear factor-?B and I?B-? in primary cultured murine brain glial cells following oxygen-glucose deprivation/ reoxygenation(OGD/R).Methods Cultured mouse brain glial cells in vitro and established OGD/R model.Glial cells were divided into five groups normal control(NC)group,OGD 24 h/R 24 h group,l-NBP 20 ?mol/L group,l-NBP 100 ?mol/L group,l-NBP 500 ?mol/L group.The protein expression of NF-?B in nuclear and I?B-? in endochylema were analyzed by Western blot.Results Comparing with NC group,OGD 24 h/R 24 h group showed a significantly higher expression of NF-?B(P