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Effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53)2007.
Article in Chinese | WPRIM | ID: wpr-592040
ABSTRACT

BACKGROUND:

Previous study reveals that,glycated albumin plays an important role on the apoptosis of endothelial cells and the expression of monocyte chemoattractant protein-1 through increasing the activity of mitogen activated protein kinase and protein tyrosine kinase,besides generating active oxygen products.

OBJECTIVE:

To investigate the effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in the cultured human umbilical vein endothelial cells(HUVECs) . DESIGN,TIME AND

SETTING:

A single sample observation was carried out in the Cardiovascular Laboratory of Southeast University(Nanjing,Jiangsu,China) from May to November in 2006. MATERIALSECV304 HUVEC strain was provided from Shanghai Institute of Cell Biology,Chinese Academy of Sciences(China) .

METHODS:

Experimental procedures were assigned to two parts. On one hand,HUVECs were cultured with glycated albumin of the concentration of 400 mg/L for 0,8,16,24,48,or 72 hours. On the other hand,HUVECs were cultured with glycated albumin of the concentrations of 100,200,400,and 800 mg/L for 24 hours. In the control group,HUVECs were incubated with bovine serum albumin of the concentration of 400 mg/L and RPMI 1640 culture medium without addition of the serum. MAIN OUTCOME

MEASURES:

The proliferation of HUVECs was estimated by MTT colorimetric assay. The content of monocyte chemoattractant protein-1 in the supernatant was measured by enzyme-linked immunosorbent assay.

RESULTS:

Glycated albumin inhibited the proliferation rate of HUVECs in a time-dependent manner(P
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2007 Type: Article