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Effects of TIMP-3 gene transient expression on proliferation,migration and apoptosis of rabbit vascular smooth muscle cells / 基础医学与临床
Basic & Clinical Medicine ; (12)2006.
Article in Chinese | WPRIM | ID: wpr-592057
ABSTRACT
Objective Observe effects of TIMP-3 gene transient expression on proliferation,migration and apoptosis of VSMCs.Methods Transfect VSMCs separately in three groups as control,empty plasmid and recombinant group mediated by cationic liposome.First,calculate cells,quantify MMP-2 and analyse TIMP-3 mRNA at 24,48 and 72 h;Second,measure cell apoptosis rate and calculate migrated cells as well after 48 h.Results There were no difference between control group and empty plasmid group in cell growth,MMP-2's content,TIMP-3 mRNA expression and apoptosis rate,cells migration rate;but those of recombinant group were greatly different in other two(P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Basic & Clinical Medicine Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Basic & Clinical Medicine Year: 2006 Type: Article