A Simple Reliable Fluorescent Assay Method for Histone Acetyltransferase (HAT) Inhibitors / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-592647
ABSTRACT
Histone acetyltransferases (HATs) are involved in the regulation of gene transcription in eukaryotic cells and their inhibitors could be a promising class of drugs due to their ability to modulate transcription and exert antiviral, anti-inflammatory as well as antioxidant effects. Nonradioactive spectrophotometric HAT assay is an alternative method to the widespread radioactive assay but suffers from drawbacks as lack of sensitivity and accuracy. A simple, non-radioactive fluorescent assay that measures the production of CoASH was established by its facile reaction with O-phthalaldehyde and 2-amino-ethanol. This method gives much higher accuracy compared to spectrophotometric assay, and allows screening of various compounds with potential HAT inhibition. The novel assay should be a valuable tool in transcriptional research and especially drug discovery.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Progress in Biochemistry and Biophysics
Year:
2006
Type:
Article
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