The study on protein expressions of IL-18,ICAM-1 and TNF-? in glomeruli of diabetic rat / 中国糖尿病杂志

ABSTRACT

Objective To study the effect of inflammatory cytokines on diabetic nephropathy.Methods Blood glucose(BG),blood urea nitrogen(BUN),serum creatinine(Scr) and 24 hour urinary protein(24hUPro) were detected at the end of 4 weeks and 8 weeks study in male SD rats.The rats were divided into diabetes(DM) group and normal control(NC) group.Kidney weight/body weight ratio(KI) of rats were measured and calculated.Kidney pathological changes were observed by light microscope and electron microscope.Expressions of interleukin-18(IL-18),intercellular adhesion molecule-1(ICAM-1)and tumor necrosis factor-alpha(TNF-?) in glomeruli were detected by immunohistochemical staining.Results Compared with homochronous NC group,BG,KI,24hUPro,Scr and expressions of IL-18,ICAM-1,TNF-? were significantly higher in DM group(P