Experimental Study of Preparation and Characteristics of Biomaterials Fabricating Esophageal Prosthesis / 医疗卫生装备

ABSTRACT

Objective To investigate the characteristics and cytocomptibility of porcine aorta fixed with genipin in vitro and to provide the proper biomaterials for fabrication of esophageal prosthesis. Methods The porcine aortas were treated by genipin for 7min to 7days, were then examined with naked-eye and light microscope. Subsequently, the fixation index determination and the biomechanics test were also performed. The cytotoxicity of the genipin-fixed materials was evaluated in vitro by MTT assay using a mouse -derived established cell line of L-929 fibroblasts as test cells. Results The experimental results indicated that the antigenicity of porcine aorta tissues could be diminished by genipin through getting rid of cell in the porcine aorta tissues and reducing the level of free amino groups in the porcine aorta tissues. The structural integrity of porcine aorta tissues can be preserved after treating with genipin. It was also found that the genipin-fixed porcine aorta tissues appeared more toughness and visco-elasticity. The RGR of genipin-fixed porcine aorta tissues was high which presumed a low cytotoxicity of this material. Conclusion The Genipin-fixed porcine aorta should be a promising material for fabricating esophageal prosthesis or fabricating scaffold of tissue-engineered esophagus.