Inhibitory effect of 4'-methylether-scutellarein on human choriocarcinoma JAR cells and the possible mechanism / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-592836
ABSTRACT
Objective:
To investigate the inhibitory effect of 4'-methylether-scutellarein(4-MS),an extract from Verbena officinalis,on human choriocarcinoma JAR cell line and the possible mechanism.Methods:
JAR cells were exposed to 4'-methylether-scutellarein of different concentrations for 48 h.MTT assay was used to examine the anti-proliferative effect of 4'-methylether-scutellarein.Flow cytometry was used to investigate the apoptosis and the changes of cell cycle.AO/EB double staining was applied to discriminate the apoptotic cells from dead ones.The changes of Survivin,p38-MAPK and Caspase 3 mRNA expressions were detected by RT-PCR in JAR cells treated with 4-MS.Furthermore,Western blotting assay was used to determine Survivin protein expression,phosphorylation level of p38 and Caspase 3 in JAR cells before and after 4-MS treatment.Results:
4-MS inhibited the proliferation of JAR cells in a dose- and time-dependent manner(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2006
Type:
Article
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