Effects of Tianshu capsule on the plasma levels of ?-endorphin and 5-Hydroxytryptamine and c-fos expression of brain tissue in rats with migraine / 临床神经病学杂志

ABSTRACT

Objective To investigate the effects of Tianshu capsule(TSC) on the plasma levels of ?-endorphin(?-EP) and 5-Hydroxytryptamine(5-HT) and c-fos expression of brain tissue in the rat with migraine.Methods The rat migraine model was established by subcutaneously administration of nitroglycerin.The intragastric administration dose of TSC was divided into three groups as followshigh dose(7.5 g/kg),middle dose(3.75 g/kg) and low dose(1.88 g/kg).TSC was administrated respectively in therapeutic and prophylactic model.The control group and model group were administrated corresponding dose of normal saline.The plasma levels of ?-EP and 5-HT in rat were measured by radioimmunoassay and HPLC respectively;the distribution of ?-EP,5-HT and c-fos in rats periaqueductal grey were evaluated by immunohistochemistry.Results Compared to model group,the plasma levels of ?-EP were increased remarkably in high dose TSC therapeutic and prophylactic groups,middle dose prophylactic group(all P