Progress of in vivo use of RNA interference / 基础医学与临床

ABSTRACT

RNA interference(RNAi),the first method of choice to suppress gene expression in vitro,is a conserved biologic response to double-stranded RNA that resulted in the sequence-specific silencing of target gene expression.Recently,more than 100 literatures reported synthetic small interfering RNAs in mammals.These reports demonstrated that RNAi is also a powerful tool for in vivo research and may become an effective therapeutic tool.This paper reviews the influencing factors and experimental design of in vivo use of RNAi.