Protective effects of estrogen on brain injured by cerebral ischemia-reperfusion in ovariectomized rats / 临床神经病学杂志

ABSTRACT

Objective To study the protective effects of estrogen on brain injured by cerebral ischemia-reperfusion in ovariectomized rats.Methods 30 d after bilateral ovariectomy,the benzestrofol 100 ?g/(kg?d)were intramusculari injected in to the models of ovariectomied rats for 14 d. Then the rat models of focal cerebral ischemia-reperfusion were made. The expressions of CD54 and TNF-? in brain tissue were detected by immunohistochemistry. The apoptotic cells were assayed by TUNEL,and the ultramicrostructural changes of neuron membrane was observed by electron microscope. Results Compared with the ischemia-reperfusion group and ovariectomized group,the expressions of CD54 and TNF-? of brain tissue in the estrogen group were significantly lower,and the apoptosis was reduced (all P