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Identification of scar animal models with method of picric-sirius red polarized light / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53): 182-183,插5, 2006.
Article in Chinese | WPRIM | ID: wpr-593400
ABSTRACT

BACKGROUND:

Stable reliable experimental animal models are needed urgently in scar research.

OBJECTIVE:

Scar animal models of nude mice are evaluated with histological method to define optimal opportunity for using.

DESIGN:

Randomizly controlled and repetitively measured design.

SETTING:

Department of Burn, First Affiliated Hospital, Sun Yat-sen University.MATERIALS The experiment was conducted at the Center for Animal Experiment, Medical College, Sun Yat-sen University between January 2004 and March 2004. Fifteen nude mice aged 4-6 weeks were provided by Center for Animal Experiment, Medical College, Sun Yat-sen University (of either gender with body mass of 15-25 g). Hyperplastic scar was gained from samples of exairesis in patients with burn after healing which is hyperplastic scar for half a year.

METHODS:

Human hyperplastic scar was grafted at dorsa of nude mice to establish scar animal models. After graft for four weeks, 5 experimental animals were killed every week, and grafts were gained. 100 g/L formalin was used to fix samples for 3 weeks. Picric-sirius red polarized light method was used to detect the graft and clinical materials, and histological feature was observed.MAIN OUTCOME

MEASURES:

①Results of film reading of picric-sirius red polarized light method. ②Analytic result of computer image.

RESULTS:

①Results of film reading of picric-sirius red polarized light

method:

The grafts showed the same feature of diffused distribution of mainly yellow and red thick fiber with thin-mesh green fiber under polarized light in every time segment group. ②Analytic result of computer image In clinicopathological hyperplastic scar, type Ⅰ collagen was about 74%; type Ⅲ collagen accounted for about 26%. In the graft from 4-6 weeks, the contents of type Ⅰ collagen were (74.52 ±0.47)% , (74.43 ±0.53)% ,(74.69±0.63)%, respectively; The contents of type Ⅲ were (25.48±0.47)%, (25.57±0.53)%, (25.31±0.63)%, respectively, which had insignificant difference (P > 0.05 ).

CONCLUSION:

In the time segment designed by experiment, the feature of graft and clinical material is coincident, which is accorded with the characteristics of hyperplastic scar. The detection of collagen of scar tissue with picric-sirius red polarized light method is a simple effective method for assessing the tissue of hyperplastic scar. Establishing scar models with nude mice is effective and stable.
Full text: Available Index: WPRIM (Western Pacific) Type of study: Controlled clinical trial / Diagnostic study / Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Controlled clinical trial / Diagnostic study / Prognostic study Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2006 Type: Article