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Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury
Korean Circulation Journal ; : 193-200, 2017.
Article in English | WPRIM | ID: wpr-59345
ABSTRACT
BACKGROUND AND

OBJECTIVES:

The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. MATERIALS AND

METHODS:

We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial H2O2 and mitochondrial complex activity.

RESULTS:

KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress.

CONCLUSION:

KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Potassium Cyanide / Reperfusion Injury / Down-Regulation / Reactive Oxygen Species / Electron Transport Complex IV / Oxidative Stress / Cytochromes / Cytochromes c / Infarction / Ischemia Limits: Animals Language: English Journal: Korean Circulation Journal Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Potassium Cyanide / Reperfusion Injury / Down-Regulation / Reactive Oxygen Species / Electron Transport Complex IV / Oxidative Stress / Cytochromes / Cytochromes c / Infarction / Ischemia Limits: Animals Language: English Journal: Korean Circulation Journal Year: 2017 Type: Article