Relationship between P53 and survivin in human lung cancer cells / 中国肿瘤生物治疗杂志

ABSTRACT

Objective:To observe the inhibitory effects of survivin-specific siRNA on human lung cancer cell lines with different P53 statuses,and to analyse the relationship between P53 and survivin in vitro.Methods:A549(wild-type P53)and H1299(P53 absent)cells were transfected with chemosynthetic survivin siRNA.The effect of siRNA on cell proliferation was analysed by MTT assay.The changes of cell cycle and apoptosis were examined by flow cytometry.The expression of survivin mRNA was detected by RT-PCR.The protein expression of survivin,P53,P21 and PARP was examined by Western blotting.Results:Endogenous survivin level in A549 cells was lower than that in H1299 cells.The mRNA and protein expression of survivin was significantly lower in siRNA group than in blank control group and non-silencing siRNA group(P