Anti-tumor and hypoxia regulatory effect of HIF-1 a specific siRNA in transplanted VX2 tumor / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)2006.
Article
in Chinese
| WPRIM
| ID: wpr-593539
ABSTRACT
Objective:
To explore the anti-tumor and hypoxia regulatory effect of HIF-1?siRNA in tumor tissues.Methods:
The HIF-1?siRNA adenovirus vectors targeting HIF-1?were constructed and the rabbit VX2 tumor model was established.The constructed vectors were injected into rabbits through ear vein;noninterference sequence was injected as control.PET(positron emission computed tomography)was used to observe the changes of hypoxia and necrosis in tumor tissues.The tumors were removed and the tumor volumes were measured.H-E staining was used to observe the degree of the necrosis;RT-PCR was used to detect the change of HIF-1?expression in tumor tissues.Results:
The recombinant HIF-1?siRNA adenovirus vectors were successfully constructed,with a titre of 1.1?10~(10)pfu/mL.PET imaging showed that the SUV of ~(18)F-FDG was significantly decreased in HIF-1?siRNA injected group than that in the control group ([3.51?0.36]%vs[8.73?0.83]%,P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2006
Type:
Article
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