Expression of vascular endothelial growth factor and transforming growth factor-beta 1 in synovial membrane of osteoarthritis and their relationships

Ningjie CHEN

ABSTRACT

BACKGROUND:

The vascular endothelial growth factor (VEGF) and transforming growth factor-?1 (TGF-?1) are the family members of the synthesis and decomposition class cell factors, which closely relate to osteoarthritis. But the expression of VEGF and TGF-?1 in previous reports mainly focuses on the cartilage cell and matrix aspect in osteoarthritis is superficial, especially in synovial membrane tissues.

OBJECTIVE:

To further understand the expression of VEGF and TGF-?1 in the synovial membrane of osteoarthritis, and to study the relationship between the two factors. DESIGN, TIME AND

SETTING:

The in vitro cytology experiment was completed in the Molecular Biology Laboratory of Zibo Central Hospital, Affiliated Hospital of Binzhou Medical College from January 2003 to September 2004.

PARTICIPANTS:

A total of 30 pathology specimen of synovial membrane which conformed to the diagnoses standard of osteoarthritis, and 8 pathology specimen of synovial membrane which were unnatural death or traumatic amputation healthy people were selected.

METHODS:

All synovial membrane specimen were performed immunohistochemistry staining, peroxidase solution, normal horse serum, first antibody, polyvalent biotinylated antibody, streptavidin-peroxydase solution, and DAB solution were added orderly, then followed by haematoxylin staining. In the control group, all procedure was the same as the synovial membrane instead of the first antibody was replaced with PBS. MAIN OUTCOME

MEASURES:

The expression of VEGF and TGF-?1 in the synovial membrane and their relationship.

RESULTS:

The positive expression rates of VEGF and TGF-?1 were 86.7% and 13.3%, respectively. Compared with normal tissues, the positive expression rate of TGF-?1 was lower (P