Transdifferentiation of rat muscle derived stem cells into insulin-secreting cells in vitro / 基础医学与临床

ABSTRACT

Objective To explore the potential diferentiation of insulin-secreting cells from muscle derived stem cells.Methods SD rat muscle derived stem cells(MDSCs)were isolated and then induced for 7～12 days with combination inductor.Morphological changes of the induced cells were observed under an inveted microscope.DTZ staining,immunocytochemical staining were conducted to examine the production of insulin.Glucose challenge and radio immunoassay were perfomed to evaluate the function of the aggregates,and RT-PCR was conducted to detect the expression of endocrine gene.Results Typical insulin-producing cells were observed at the end of the protocol(12 days).Ditizone staining and immunohistochemistry for insulin were both positive.Insulin was secreted by these cells in response to different concentrations of glucose stimulation(P