Effect of AdIL-12 infected mesenchymal stem cells on proliferation of C6 glioma cells / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1994.
Article
in Chinese
| WPRIM
| ID: wpr-593890
ABSTRACT
Objective:
To study the influence of mesenchymal stem cells(MSC) infected by AdIL-12 on the proliferation of C6 glioma cells.Methods:
The MSCs were cultured from rat bone marrow and verified by immunohistochemistry and flow cytometry.Recombinant adenovirus vectors harboring IL-12(AdEasy-IL-12) were infected into MSCs to construct AdIL-12-MSC containing exogenous IL-12.RT-PCR and Western Blotting were used to detect IL-12 mRNA and protein expression in AdIL-12-MSC.MTT method was used to detect the effect of AdIL-12-MSC supernatant on the proliferation of C6 glioma cells.Results:
Exogenous IL-12 gene was effectively transfected into MSCs by recombinant adenovirus vectors.IL-12 was expressed in MSCs at both mRNA and protein levels as detected by RT-PCR and Western Blotting.The supernatant of AdIL-12-MSC significantly inhibited the proliferation of C6 glioma cells compared with MSC supernatant(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1994
Type:
Article
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