Adenosine A_1 Receptor Agonist Prevents Angiotensin Ⅱ Induced Hypertrophy in Cultured Myocardial Cells of Neonatal Rats / 中华高血压杂志

ABSTRACT

Objective To demonstrate the inhibitory effect of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropy1)adenosine(R-PIA)and cross-talk between adenosine A1 receptor and CaN in cultured myocardial cells hypertrophy induced by Ang Ⅱ.Methods The protein synthetic rate was determined by measuring the incorporation of [3H]-leucine into myocyte protein;the cardiomyocytes volumes were measured by computer photograph analysis system;the expression of atrial natriuretic polypeptide(ANP)and CaN were determined by Western-blot.Results R-PIA(1 ?mol/L)inhibited Ang Ⅱ(0.1 ?mol/L)-induced increase of [3H]-leucine incorporation [R-PIA(976.2?89.0)vs Ang Ⅱ(1130.7?102.7)count per min per well,P