Distribution of Bactericidal/permeability Increasing Protein mRNA in Rat / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate systematically the existence of bactericidal/permeability increasing protein(BPI) in normal rat organs and tissues,for providing important information about BPI in clinical application.METHODS The total RNA was extracted from organs and tissues homogenates.Then the first-strand cDNA was synthesized and BPI DNA was amplified by PCR.RESULTS BPI mRNA was found in kidneys,ovary,testes,liver,small intestine,large intestine,thymus,spleen and neutrophils of 21 various organs and tissues.Among them,BPI mRNA content was the richest in testes,relatively rich in liver and large intestine.CONCLUSIONS BPI mRNA presents in many organs and tissues of rat and it indicates that the role of BPI in host defense against bacterial infection is relatively widespread.