Isolation,culture and oriented differentiation of bone marrow mesenchymal stem cells / 中国组织工程研究

ABSTRACT

Bone marrow mesenchymal stem cells(BMSCs) are a kind of non-hematopoietic cells living among bone marrow stroma,and are able to differentiate into mesoderm-type cells and non-mesoderm type cells.At present,there are three main methods for isolating and purifying BMSCsbone marrow adherent culture,density gradient centrifugation and immune selection.This review listed many kinds of cells that BMSCs can differentiate into under certain conditions,discussed the related different directing conditions reported by researchers,analyzed the principle behind the artificial controlling conditions,and tried to find relatively uniform and effective directing conditions.The cells that BMSCs can differentiate into various types of cells including mesoderm-type cells,and non-mesoderm type cellsosteocytes,chondrocytes,adiocytes.As seed cells and a source of cell therapy in tissue engineering,BMSCs have been widely used in studying stem cells.