Suppression of growth of micrangium of gastric cancer cells xenograft in nude mice by VEGF antisense oligonucleotide / 基础医学与临床

ABSTRACT

Objective To observe the effects of VEGF antisense oligonucleotide on growth of micrangium of xenograft in nude mice.Methods VEGF-ASODN was synthesised.After transfection with VEGF-ASODN,RNA copy was detected by real-time PT-PCR,VEGF protein was examined by ELESA,Survival of cells was tested by MTT.Growth of cells was shown by growth curve.After the model of human gastic cancer xenografts in nude mice was developed,model animals were divided randomly into three groupsantisense group、scrambled group and saline group.ResultsVEGF-ASODN can reduce the VEGF mRNA and VEGF protein in cells and supernate remarkably.Survival and growth of cells were suppressed.It can significantly reduce VEGF protein in serum of nude mice.It also reduced the volum and weight of xenografts.Density of micrangium decreased in xenografts.Conclusion VEGF-ASODN can suppress the growth of micrangium in xenografts.