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Specific gene expression of tissue engineered periosteum constructed by different original osteoblasts / 中国组织工程研究
Chinese Journal of Tissue Engineering Research ; (53)2007.
Article in Chinese | WPRIM | ID: wpr-594672
ABSTRACT

BACKGROUND:

Seed cells from different sources have different ability in cell adhesion,proliferation,and differentiation,which can led to bioactive diversity in constructed tissue engineered products.

OBJECTIVE:

To explore the differentiation ability of different original fetal osteoblasts during constructing tissue engineered periosteum at molecular level. DESIGN,TIME AND

SETTING:

The contrast observation was performed at the Central Laboratory of Shaanxi Provincial Peoples’ Hospital between July 2007 and July 2008. MATERIALSThe human amnion cells(consent was obtained from the puerperant) were prepared human acellular amniotic membrane(HAAM) .

METHODS:

Periosteum-origin osteoblasts(POB) and cranium-origin osteoblasts(COB) were seed on HAAM,cultured for 2,4,6,8,and 10 days,and then their total RNA was extracted,which were reversely transcripted to cDNA. The real-time PCR analysis was used to reveal core binding factor ?l(Cbfa1) ,Osterix,and the cycle threshold was also measured. MAIN OUTCOME

MEASURES:

The expression of Cbfa1,Osterix,as well as osteocalcin.

RESULTS:

On tissue engineered periosteum,the expression of Cbfa1 in POB was lower than it that in COB(P
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Tissue Engineering Research Year: 2007 Type: Article