Decalcified bone matrix and bone cement compound at various proportions in repairing rabbit femoral defect / 中国组织工程研究

ABSTRACT

BACKGROUND:BACKGROUND:Present studies have shown that bone cement have less osteoinduction and slow degradation in the body,so the effect of alone application is not satisfactory. Therefore,people have modified them,hoping to research a new materials that can overcome all shortcomings above-mentioned. OBJECTIVE:To investigate the ability of decalcified bone matrix (DBM) and acrylic bone cement (ABC) compound in filling and repairing bone defect,and to determine the best component proportion of the composition. DESIGN,TIME AND SETTING:Randomized controlled animal experiments were performed at the Experimental Animal Center,Chongqing Medical University from May to September 2008. MATERIALSABC and DBM were prepared into compound material at various proportions (28,37,46,55,64). METHODS:The animal models of bilateral femur bone defects and fillers were established in the New Zealand White rabbits. The complex material was implanted on the bone defect; the ABC was used as blank control group. MAIN OUTCOME MEASURES:The complex and alone materials were examined by biomechanics and scan electron microscopes. Gross observation,histopathological and X-ray test were used at 4,8,12 weeks after operation respectively to observe the repaired and filled status of bone defect. RESULTS:DBM composite quality of the scope of 37 -64,the composite materials that existed in more cracks more than 100 ?m,when the DBM was less than 37,the material inside the aperture and the majority of material was similar to ABC,mass ratio greater than 64 when the DBM and the ABC could not effectively together solidification. When the mass ratio was increased,which matrix particles of demineralized bone and ABC,the compressive ultimate strength of composite materials was less than pure ABC (P